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Fig. 4. Relative mRNA levels of selected genes related to bile acid metab- olism in the liver and small intestine of control mice (A) and disodium ascor- byl phytostanol phosphate (FM-VP4)-fed mice (g). mRNA levels were quantified by real-time RT-PCR using glyceraldehyde 3-phosphate dehydro- genase as an internal control. CYP7A1, cytochrome P450 family 7 subfamily A polypeptide 1; FXR, farnesoid X receptor; BSEP, bile salt export pump; <t>NTCP,</t> Naþ/taurocholate co-transporter polypeptide; IBABP, ileal bile acid binding protein. The box-and-whisker graphs show the median (of five mice per group) as the middle line. The box extends from the 25th to the 75th per- centile and the whiskers extend from the lowest value to the highest. Medians of control values were set at a normalised value of 100 arbitrary units. W, Outside values; *, far-out values. † Median value was significantly different from that of the control mice (P,0·05).
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Fig. 4. Relative mRNA levels of selected genes related to bile acid metab- olism in the liver and small intestine of control mice (A) and disodium ascor- byl phytostanol phosphate (FM-VP4)-fed mice (g). mRNA levels were quantified by real-time RT-PCR using glyceraldehyde 3-phosphate dehydro- genase as an internal control. CYP7A1, cytochrome P450 family 7 subfamily A polypeptide 1; FXR, farnesoid X receptor; BSEP, bile salt export pump; <t>NTCP,</t> Naþ/taurocholate co-transporter polypeptide; IBABP, ileal bile acid binding protein. The box-and-whisker graphs show the median (of five mice per group) as the middle line. The box extends from the 25th to the 75th per- centile and the whiskers extend from the lowest value to the highest. Medians of control values were set at a normalised value of 100 arbitrary units. W, Outside values; *, far-out values. † Median value was significantly different from that of the control mice (P,0·05).
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Johns Hopkins HealthCare human protein reference database
Fig. 4. Relative mRNA levels of selected genes related to bile acid metab- olism in the liver and small intestine of control mice (A) and disodium ascor- byl phytostanol phosphate (FM-VP4)-fed mice (g). mRNA levels were quantified by real-time RT-PCR using glyceraldehyde 3-phosphate dehydro- genase as an internal control. CYP7A1, cytochrome P450 family 7 subfamily A polypeptide 1; FXR, farnesoid X receptor; BSEP, bile salt export pump; <t>NTCP,</t> Naþ/taurocholate co-transporter polypeptide; IBABP, ileal bile acid binding protein. The box-and-whisker graphs show the median (of five mice per group) as the middle line. The box extends from the 25th to the 75th per- centile and the whiskers extend from the lowest value to the highest. Medians of control values were set at a normalised value of 100 arbitrary units. W, Outside values; *, far-out values. † Median value was significantly different from that of the control mice (P,0·05).
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TaKaRa dsred monomer protein fusions
Fig. 4. Relative mRNA levels of selected genes related to bile acid metab- olism in the liver and small intestine of control mice (A) and disodium ascor- byl phytostanol phosphate (FM-VP4)-fed mice (g). mRNA levels were quantified by real-time RT-PCR using glyceraldehyde 3-phosphate dehydro- genase as an internal control. CYP7A1, cytochrome P450 family 7 subfamily A polypeptide 1; FXR, farnesoid X receptor; BSEP, bile salt export pump; <t>NTCP,</t> Naþ/taurocholate co-transporter polypeptide; IBABP, ileal bile acid binding protein. The box-and-whisker graphs show the median (of five mice per group) as the middle line. The box extends from the 25th to the 75th per- centile and the whiskers extend from the lowest value to the highest. Medians of control values were set at a normalised value of 100 arbitrary units. W, Outside values; *, far-out values. † Median value was significantly different from that of the control mice (P,0·05).
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Fig. 4. Relative mRNA levels of selected genes related to bile acid metab- olism in the liver and small intestine of control mice (A) and disodium ascor- byl phytostanol phosphate (FM-VP4)-fed mice (g). mRNA levels were quantified by real-time RT-PCR using glyceraldehyde 3-phosphate dehydro- genase as an internal control. CYP7A1, cytochrome P450 family 7 subfamily A polypeptide 1; FXR, farnesoid X receptor; BSEP, bile salt export pump; <t>NTCP,</t> Naþ/taurocholate co-transporter polypeptide; IBABP, ileal bile acid binding protein. The box-and-whisker graphs show the median (of five mice per group) as the middle line. The box extends from the 25th to the 75th per- centile and the whiskers extend from the lowest value to the highest. Medians of control values were set at a normalised value of 100 arbitrary units. W, Outside values; *, far-out values. † Median value was significantly different from that of the control mice (P,0·05).
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Fig. 4. Relative mRNA levels of selected genes related to bile acid metab- olism in the liver and small intestine of control mice (A) and disodium ascor- byl phytostanol phosphate (FM-VP4)-fed mice (g). mRNA levels were quantified by real-time RT-PCR using glyceraldehyde 3-phosphate dehydro- genase as an internal control. CYP7A1, cytochrome P450 family 7 subfamily A polypeptide 1; FXR, farnesoid X receptor; BSEP, bile salt export pump; <t>NTCP,</t> Naþ/taurocholate co-transporter polypeptide; IBABP, ileal bile acid binding protein. The box-and-whisker graphs show the median (of five mice per group) as the middle line. The box extends from the 25th to the 75th per- centile and the whiskers extend from the lowest value to the highest. Medians of control values were set at a normalised value of 100 arbitrary units. W, Outside values; *, far-out values. † Median value was significantly different from that of the control mice (P,0·05).
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Fig. 4. Relative mRNA levels of selected genes related to bile acid metab- olism in the liver and small intestine of control mice (A) and disodium ascor- byl phytostanol phosphate (FM-VP4)-fed mice (g). mRNA levels were quantified by real-time RT-PCR using glyceraldehyde 3-phosphate dehydro- genase as an internal control. CYP7A1, cytochrome P450 family 7 subfamily A polypeptide 1; FXR, farnesoid X receptor; BSEP, bile salt export pump; <t>NTCP,</t> Naþ/taurocholate co-transporter polypeptide; IBABP, ileal bile acid binding protein. The box-and-whisker graphs show the median (of five mice per group) as the middle line. The box extends from the 25th to the 75th per- centile and the whiskers extend from the lowest value to the highest. Medians of control values were set at a normalised value of 100 arbitrary units. W, Outside values; *, far-out values. † Median value was significantly different from that of the control mice (P,0·05).
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Transduction Laboratories Inc 7d3 rat anti-human cytokeratin monoclonal antibody
Fig. 4. Relative mRNA levels of selected genes related to bile acid metab- olism in the liver and small intestine of control mice (A) and disodium ascor- byl phytostanol phosphate (FM-VP4)-fed mice (g). mRNA levels were quantified by real-time RT-PCR using glyceraldehyde 3-phosphate dehydro- genase as an internal control. CYP7A1, cytochrome P450 family 7 subfamily A polypeptide 1; FXR, farnesoid X receptor; BSEP, bile salt export pump; <t>NTCP,</t> Naþ/taurocholate co-transporter polypeptide; IBABP, ileal bile acid binding protein. The box-and-whisker graphs show the median (of five mice per group) as the middle line. The box extends from the 25th to the 75th per- centile and the whiskers extend from the lowest value to the highest. Medians of control values were set at a normalised value of 100 arbitrary units. W, Outside values; *, far-out values. † Median value was significantly different from that of the control mice (P,0·05).
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Novocastra rabbit polyclonal antibody to ubiquitin
Fig. 4. Relative mRNA levels of selected genes related to bile acid metab- olism in the liver and small intestine of control mice (A) and disodium ascor- byl phytostanol phosphate (FM-VP4)-fed mice (g). mRNA levels were quantified by real-time RT-PCR using glyceraldehyde 3-phosphate dehydro- genase as an internal control. CYP7A1, cytochrome P450 family 7 subfamily A polypeptide 1; FXR, farnesoid X receptor; BSEP, bile salt export pump; <t>NTCP,</t> Naþ/taurocholate co-transporter polypeptide; IBABP, ileal bile acid binding protein. The box-and-whisker graphs show the median (of five mice per group) as the middle line. The box extends from the 25th to the 75th per- centile and the whiskers extend from the lowest value to the highest. Medians of control values were set at a normalised value of 100 arbitrary units. W, Outside values; *, far-out values. † Median value was significantly different from that of the control mice (P,0·05).
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Biogen Inc recombinant human iia secretory phospholipase a 2 (spla 2)
Fig. 4. Relative mRNA levels of selected genes related to bile acid metab- olism in the liver and small intestine of control mice (A) and disodium ascor- byl phytostanol phosphate (FM-VP4)-fed mice (g). mRNA levels were quantified by real-time RT-PCR using glyceraldehyde 3-phosphate dehydro- genase as an internal control. CYP7A1, cytochrome P450 family 7 subfamily A polypeptide 1; FXR, farnesoid X receptor; BSEP, bile salt export pump; <t>NTCP,</t> Naþ/taurocholate co-transporter polypeptide; IBABP, ileal bile acid binding protein. The box-and-whisker graphs show the median (of five mice per group) as the middle line. The box extends from the 25th to the 75th per- centile and the whiskers extend from the lowest value to the highest. Medians of control values were set at a normalised value of 100 arbitrary units. W, Outside values; *, far-out values. † Median value was significantly different from that of the control mice (P,0·05).
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Fig. 4. Relative mRNA levels of selected genes related to bile acid metab- olism in the liver and small intestine of control mice (A) and disodium ascor- byl phytostanol phosphate (FM-VP4)-fed mice (g). mRNA levels were quantified by real-time RT-PCR using glyceraldehyde 3-phosphate dehydro- genase as an internal control. CYP7A1, cytochrome P450 family 7 subfamily A polypeptide 1; FXR, farnesoid X receptor; BSEP, bile salt export pump; <t>NTCP,</t> Naþ/taurocholate co-transporter polypeptide; IBABP, ileal bile acid binding protein. The box-and-whisker graphs show the median (of five mice per group) as the middle line. The box extends from the 25th to the 75th per- centile and the whiskers extend from the lowest value to the highest. Medians of control values were set at a normalised value of 100 arbitrary units. W, Outside values; *, far-out values. † Median value was significantly different from that of the control mice (P,0·05).
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Image Search Results


Fig. 4. Relative mRNA levels of selected genes related to bile acid metab- olism in the liver and small intestine of control mice (A) and disodium ascor- byl phytostanol phosphate (FM-VP4)-fed mice (g). mRNA levels were quantified by real-time RT-PCR using glyceraldehyde 3-phosphate dehydro- genase as an internal control. CYP7A1, cytochrome P450 family 7 subfamily A polypeptide 1; FXR, farnesoid X receptor; BSEP, bile salt export pump; NTCP, Naþ/taurocholate co-transporter polypeptide; IBABP, ileal bile acid binding protein. The box-and-whisker graphs show the median (of five mice per group) as the middle line. The box extends from the 25th to the 75th per- centile and the whiskers extend from the lowest value to the highest. Medians of control values were set at a normalised value of 100 arbitrary units. W, Outside values; *, far-out values. † Median value was significantly different from that of the control mice (P,0·05).

Journal: British Journal of Nutrition

Article Title: Disodium ascorbyl phytostanol phosphate (FM-VP4), a modified phytostanol, is a highly active hypocholesterolaemic agent that affects the enterohepatic circulation of both cholesterol and bile acids in mice

doi: 10.1017/s0007114509991656

Figure Lengend Snippet: Fig. 4. Relative mRNA levels of selected genes related to bile acid metab- olism in the liver and small intestine of control mice (A) and disodium ascor- byl phytostanol phosphate (FM-VP4)-fed mice (g). mRNA levels were quantified by real-time RT-PCR using glyceraldehyde 3-phosphate dehydro- genase as an internal control. CYP7A1, cytochrome P450 family 7 subfamily A polypeptide 1; FXR, farnesoid X receptor; BSEP, bile salt export pump; NTCP, Naþ/taurocholate co-transporter polypeptide; IBABP, ileal bile acid binding protein. The box-and-whisker graphs show the median (of five mice per group) as the middle line. The box extends from the 25th to the 75th per- centile and the whiskers extend from the lowest value to the highest. Medians of control values were set at a normalised value of 100 arbitrary units. W, Outside values; *, far-out values. † Median value was significantly different from that of the control mice (P,0·05).

Article Snippet: Primers were obtained from Applied Biosystems databases (references: liver X receptor (LXR)-a: Mm00443450_m1; ABCG5: Mm00446243_m1; ABCG8: Mm00445980_m1; ABCA1: Mm00442649_m1; HMGCoA-R: 1579156A; NPC1L1: Mm01191979_m1; scavenger receptor class BI (SR-BI): Mm00450236_m1; farnesoid X receptor (FXR): Mm00436419_m1; Naþ/taurocholate co-transporter polypeptide (NTCP): Mm00441421_m1; CYP7A1: Mm00484152_m1; bile salt export pump (BSEP): Mm00445168_m1; ileal bile acid binding protein (IBABP): Mm00434316_m1; glyceraldehyde 3-phosphate dehydrogenase (GAPDH): Mm99999915_g1).

Techniques: Control, Quantitative RT-PCR, Binding Assay, Whisker Assay